Journal: Journal of cell science

Article Title: Developmental-stage-specific triacylglycerol biosynthesis, degradation and trafficking as lipid bodies in Plasmodium falciparum-infected erythrocytes.

doi: 10.1242/jcs.00988

Figure Lengend Snippet: Fig. 1. Stage-specific incorporation of 14C-labeled oleic acid into TAG in P. falciparum-infected erythrocytes. Tightly synchronized cultures of Honduras-1 were labeled and total lipids associated with infected erythrocyte were analysed. (A) TLC of the extracted total lipid species for neutral (left) and polar (right) lipids. The positions corresponding to the authentic cold lipid species are indicated: CE, cholesteryl ester; DAG, diacylglycerol; FFA, free fatty acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; TAG, triacylglycerol. (B) Kinetics of the accumulation of various lipid species incorporated with radiolabeled oleic acid in P. falciparum-infected erythrocytes during intraerythrocytic development. At different time points, the distribution of lipid-associated radioactivity in TAG (filled circles), PC (open circles) and PE (open triangles) are shown. Values are the total radioactivity of each lipid from 5 ml of 3% hematocrit culture. At the top is shown the dominant parasite morphology at various sampling times. One of four independent experiments displaying similar profile is shown.

Article Snippet: Oleic acid, phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were from Avanti Polar Lipids (AL, USA).

Techniques: Labeling, Infection, Radioactivity, Sampling

Journal:

Article Title: Lysophosphatidic acid (LPA) and Angiogenesis

doi: 10.1007/s10456-008-9113-5

Figure Lengend Snippet: To analyze the role of LPA receptors in LPA-induced angiogenesis we used the LPA1 and LPA3 receptor antagonist, VPC32183. Using the same approach aforementioned, we show in 3A and 3B that LPA-induced angiogenesis is blocked completely by this compound at 10µM. Importantly, VPC32183 alone (at 10µM) had no discernable effect in angiogenesis and does not block VEGF-induced angiogenesis (Figure 3C). One-Way ANOVA test was performed between groups. ** P< 0.001. Error bars represent standard deviation (SD). In figure 3B (scale bar: 0.125 cm), we present the image of a single membrane representative of each group.

Article Snippet: Disclosures : VPC32183 is sold by Avanti Polar Lipids under license from the University of Virginia.

Techniques: Blocking Assay, Standard Deviation

Journal:

Article Title: Lysophosphatidic acid (LPA) and Angiogenesis

doi: 10.1007/s10456-008-9113-5

Figure Lengend Snippet: S-1-oleoyl-2-O-methylglycerophosphothionate (S-OMPT), at 100nM, is able to induce a strong angiogenic response, similar to the LPA response at this concentration (4A–4C). CAMs treated with S-OMPT (100nM) together with VPC32183 (10µM) document that the response is completely blocked by inclusion of this antagonist (4C). For statistical analysis, Student t test was performed between groups. ** P< 0.001. Error bars represent standard deviation (SD). Figure 4B is the image of a single membrane that is representative of each group. Scale bar: 0.125 cm.

Article Snippet: Disclosures : VPC32183 is sold by Avanti Polar Lipids under license from the University of Virginia.

Techniques: Concentration Assay, Standard Deviation

Journal:

Article Title: Lysophosphatidic acid (LPA) and Angiogenesis

doi: 10.1007/s10456-008-9113-5

Figure Lengend Snippet: CAMs were treated with recombinant sphingomyelinase D (SMaseD) and the number of vessels that intersected the disc was counted. This enzyme is able to induce an angiogenic response (5A) similar to the LPA response. Two catalytically inactive forms of this enzyme (H37N and H73N) were unable to induce angiogenesis (5B), suggesting that the response obtained is dependent on the catalytic activity (lyso-PLD) of the arachnid enzyme. Moreover, SMaseD-induced angiogenesis is blocked by the LPA receptor antagonist VPC32183 at 10µM (5C), suggesting that the positive response obtained is dependent on the activation of LPA1,3 receptors. One-Way ANOVA test was performed between groups. ** P< 0.001. Error bars represent standard deviation (SD).

Article Snippet: Disclosures : VPC32183 is sold by Avanti Polar Lipids under license from the University of Virginia.

Techniques: Recombinant, Activity Assay, Activation Assay, Standard Deviation

Journal: Molecules

Article Title: Structural Characterization of Unusual Fatty Acid Methyl Esters with Double and Triple Bonds Using HPLC/APCI-MS 2 with Acetonitrile In-Source Derivatization

doi: 10.3390/molecules26216468

Figure Lengend Snippet: APCI MS/MS CID spectrum of the [M + 55] + • adduct of crepenynic acid methyl ester (FAME 18:2n-6 TB ,9c); MBR = 276 + 190 − 347 = 119.

Article Snippet: The standards of crepenynic acid (99%) and punicic acid methyl ester (purity 98%) were from Larodan (Malmö, Sweden), and 9-octadecynoic acid methyl ester, 9( E ),11( E )-octadecadienoic acid methyl ester, and 9( Z ),11( Z )-octadecadienoic acid methyl ester (all 98%) were purchased from Cayman Europe (Tallinn, Estonia).

Techniques: Tandem Mass Spectroscopy